Cat.No.: ME-NU-100203

This product is for life science research only and is not intended for diagnostic use.   

Features                          

Form:                    Liquid         

Amount:                  250 nmol            

Volume:                   25 μL               

Concentration:             10 mM             

Storage Conditions:        Store below –20˚C

Shelf Life:                12 months

Component:        Biotin-16-Uridine-5'-triphosphatetetralithium salt

Description:

For biotin-16-UTP, biotin is bound to uridine triphosphate via an amide linkage. Biotin-16-UTP is a substrate for SP6, T3, and T7 RNA polymerase. It can replace UTP in the in vitro transcription reaction for RNA labeling. Linearized template DNA with T7, SP6, or T3 promoter is in vitro-transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP, and Biotin-16-UTP, respectively.

Application:

Biotin-labeled RNA can be used as a hybridization probe for:
Northern blots /Southern blots/Plaque or colony lifts

RNase protection experiments

In situ hybridization

Microarray hybridization