Cat.No.: MY180908-2000

This product is for life science research only and is not intended for diagnostic use. 

Description

MY Pfusion DNA polymerase is a genetically engineered thermostable recombinant DNA polymerase that is capable of amplifying long amplicons up to 7.5kb and reduces the error rate comparing with Taq DNA polymerase with higher fidelity.

Features

Form: Liquid

Specific Activity: 2 units/μL

Source:  E. coli

Purity :  > 99 % by SDS-PAGE

Storage: -20°C

Shelf life: 12 month

 Shipping Ice pack

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmoles of dNTPs into a polynucleotide fraction at 74°C in 30 min.

 

Component	100U kit
MY Pfusion DNA polymerase	50μL
5X HF Reaction Buffer	1000μL
5X GC Reaction Buffer	1000μL
0.1M MgCl2	500μL
DMSO 100%	500μL

Application

High-fidelity PCR

Cloning

Long or Difficult Amplification

High-throughput PCR