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Nucleic Acid Extraction Kit (magnetic beads method)

 Principle of Extraction

 

The kit uses a unique magnetic bead and buffer system. The surface of the magnetic bead is modified with special chemical groups, which have extremely strong enrichment ability for DNA/RNA under certain conditions and can reversibly release DNA/RNA when the conditions are changed to rapidly separate and purify DNA/RNA and to remove impurities like proteins as far as possible. The extracted high-quality nucleic acids can be used for serial experiments like PCR, RT-PCR, gene sequencing, COVID19 etc.

 

Performance Parameter

 

1.   Extraction Purity: A260/A280= 1.3-2.3

2.   Yield of Nucleic Acid: >90%

3.   Nucleic Acid capacity≥100ng

4.   Extracted Number: 96 samples for each run

5.   Sample Adding Amount: 100-500μL of the sample

6 AccuracyCV5%

7.  Acceptable Sample Types:  serum, plasma, cultured cells, saliva, alveolar lavage fluid, nasopharyngeal aspirate, swab samples, 

position

List of Product Composition

 

Product name

Components of Solution

Specification

48pcs/box

64pcs/box

96pcs/box

Protein

K powder

Protein K powder

 

19.2mg x 1pc

 

25.6mg x 1pc

 

19.2mg x 2pc

Protein

K solution

1mol/L THAM,100%Gly,

purified water

 

0.96ml x 1pc

 

1.28ml x 1pc

 

0.96mlx 2pc

Lysis

solution

RLT

1mol/L THAM, 5mol/L sodium100% PEG

Guanidine salt, 0.5mol/L EDTA, AITCavantinetc

500μL×12wells

4wells/box

500μL×16 wells

4wells/box

500μL×96 wells

1well/box

Washing Plate 1

(PGWT)

1mol/L THAM, 5mol/L sodium100% PEG

Guanidine salt, 0.5mol/L EDTA, AITC etc.

500μL×12wells

4wells/box

500μL×16 wells

4wells/box

500μL×96 wells

1well/box

Washing Plate 2

(GWT2)

1mol/L THAMEthanol

PN Magnetic rod

510μL×12wells

4wells/box

510μL×16 wells

4wells/box

510μL×96 wells

1well/box

Eluting Plate

TE buffer

100μL×12wells

4wells/box

100μL×16 wells

4wells/box

100μL×96 wells

1well/box

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Product Features

Simple OperationUsing Magnetic adsorption, High quality nucleic acid can be obtained without centrifugation within 45 minutes.

Safety and non-toxicity: No harmful organic solvents such as Phenol/chloroform are needed.

High quality and high quantity: Core patent guarantee high nucleic acid concentration, high purity and good integrity.

Open system: It can be used on magnetic frame and also adapted to various automated nucleic acid extraction and purification instruments.

Easy to automate: It can combine with automated instruments to save time and energy and greatly reduce human errors in experiments.

 

Product Application

The kit is composed of reagents for nucleic acid extraction (magnetic beads method), suitable for extracting genomic nucleic acid DNA and RNA of bacteria, viruses and parasites from samples. It is designed for 96-well plate automatic platforms to help dramatically improve the throughput of sample extraction. 

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