Cat.No.: MY180883-2000

Description

Taq DNA polymerase is a recombinant thermostable DNA polymerase that catalyzes DNA-template-directed extension of the 3'-end of a DNA strand and a 5´flap endonuclease activity.

Features

Form: Liquid

Specific Activity: 5 units/μL

Source:  E. coli

Purity :  > 99 % by SDS-PAGE

Storage: -20°C

Shelf life: 12 months

 Shipping Ice pack

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 15 nmol of dNTP into acid insoluble material in 30 minutes at 75°C.

 

Component	2,000U kit
Taq DNA polymerase	400μL
10X Standard Taq (Mg-free) Reaction Buffer	1000μL
0.1M MgCl2	500μL

 

Application

PCR

Multiplex PCR

Difficult templates e.g., secondary structures or GC-rich sequences

Automated PCR e.g., handling at room temperatures

Hot Start PCR up to 3kb

Hot Start RT-PCR up to 3kb

Quantitative reverse transcription PCR (RT-qPCR)

Bisulfite-specific PCR