Cat.No.: MY180908-2000
This product is for life science research only and is not intended for diagnostic use.
Description
MY Pfusion DNA polymerase is a genetically engineered thermostable recombinant DNA polymerase that is capable of amplifying long amplicons up to 7.5kb and reduces the error rate comparing with Taq DNA polymerase with higher fidelity.
Features
Form: Liquid
Specific Activity: 2 units/μL
Source: E. coli
Purity : > 99 % by SDS-PAGE
Storage: -20°C
Shelf life: 12 month
Shipping Ice pack
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmoles of dNTPs into a polynucleotide fraction at 74°C in 30 min.
Component
|
100U kit
|
MY Pfusion DNA polymerase
|
50μL
|
5X HF Reaction Buffer
|
1000μL
|
5X GC Reaction Buffer
|
1000μL
|
0.1M MgCl2
|
500μL
|
DMSO 100%
|
500μL
|
Application
High-fidelity PCR
Cloning
Long or Difficult Amplification
High-throughput PCR