Cat.No.: MY180729-100
This product is for life science research only and is not intended for diagnostic use.
Description
MY NovarIII Reverse Transcriptase is a genetically engineered recombinant M-MLV Reverse Transcriptase that reduces the activity of RNase H and improves the thermal stability. First strand cDNA can be synthesized at higher temperatures than wild-type M-MLV. This product is active even at 50-55°C, with higher specificity, higher yield, and can produce cDNA products up to 12 KB.
Features
Form: Liquid
Specific Activity: 200 units/μL
Source: E. coli
Purity : > 99 % by SDS-PAGE
Storage: -20°C
Shelf life: 12 months
Shipping Ice pack
Unit Definition
One unit incorporates 1 nmol of dTTP into acid-precipitable material in 10 min at 37°C using poly(A)•oligo(dT)16 as template-primer
Component
|
10,000U kit
|
MY Novar® RT
|
50μL
|
5X Reaction Buffer
|
1000μL
|
0.1M DTT
|
500μL
|
Application
RT-LAMP, RT-PCR, RT-qPCR